Intern
Apr 2021 - May 2021 (2 months)
Plasmid preparation, ligation, transformation, Genomic DNA extraction, total RNA extraction, RT-PCR, PCR, site-directed mutagenesis, manual and automated DNA sequencing Protein purification, FPLC, HPLC (affinity, gel filtration, ion exchange and reverse phase), preparative column chromatography, membrane filtration, dialysis, SDS-PAGE, Western blot Scanning Electron Microscopy, Confocal Microscopy