Anchal

P

Sr. Research Associate II

Pliant Therapeutics

Mar 2024 - Jun 2025 (1 year 4 months)

Executed internalization and cell spreading assays; implemented RCA to scale throughput from manual 8 to 240 automated samples weekly, preventing QC delays and enabling on-time project delivery. Conducted organoid, astrocyte, and fibroblast studies to investigate a7ẞ1 regulation; generated data that supported IND filing for a DMD program in collaboration with Lonza CRO. Developed high-content imaging assays (Operetta CLS, ZEISS LSM 900); led CRISPR-mediated TGF-ẞ knockout and engineered integrin ανβ6, ανβ1, and a8ẞ1 overexpression lines, supporting advancement of a clinical stage II program. Performed ligand-binding assays and characterized integrin expression by flow cytometry (MACSQuant), ELISA, MSD, IF, IHC. Partnered with HTS group to b

P

Sr. Research Associate I

Pliant Therapeutics

Mar 2022 - Mar 2024 (2 years 1 month)

S

Sr. Research Associate I

Sardona Therapeutics

Nov 2021 - Feb 2022 (4 months)

S

Research Associate II

Sardona Therapeutics

May 2021 - Nov 2021 (7 months)

Performed discovery and implementation of biochemical/metabolic assays for SAR; contributed as a cross-functional molecular biologist in a seed-stage, high-paced environment. Developed and executed biochemical/cell-based assays, including luminescence-based iron/cholesterol metabolite assays, for lead discovery, optimization, and target ID; characterized dose-dependent inhibition of RNA-binding molecules in TNBC, NSCLC, and CRC, generating data that secured $10M in company funding. Conducted proliferation assays in PI3K-mutant/mTORC1-activated models and siRNA transfection followed by qPCR, MSD, Western blot; isolated RNA and synthesized cDNA for downstream RT-PCR/qPCR. Coordinated with vendors to secure essential supplies in a high-paced e

Graduate Research Associate

Eastern Illinois University

Aug 2019 - May 2021 (1 year 10 months)

Conducted thesis-driven research on CES1/CES2 enzymatic activity using live-cell imaging and biochemical assays. Developed epi-fluorescent microscopy assays for CES1 visualization and probe localization. Designed and synthesized carbonate-based fluorescent probes; evaluated enzyme kinetics via spectroscopy, HPLC, and cell-based models. Performed reverse transfection in hepatocellular carcinoma lines for gene knockdown effects. Constructed plasmids using Gibson assembly; verified sequences with Sanger sequencing. Taught undergraduate lab courses and supervised students under safety protocols.

B

Staff Research Associate

Banaras Hindu University – Institute of Medical Sciences

Jan 2018 - Jul 2018 (7 months)

GCP-certified in human sample handling, including DNA/RNA/protein extraction from blood and tissue to support lab programs. Conducted chromosomal karyotyping, RT-PCR, and DNA microarray analysis to investigate gene polymorphisms in pediatric disease models.

About

Detail

Timeline

Résumé